Rubin’s work showed that the outer regions of galaxies are rotating faster than expected based on the mass of the galaxies’ visible matter. This figure, which compares the observed rotation rate of stars and gas in M33 (white line) to the expected rotation rate (gray line) means the galaxy has more mass than indicated by its visible matter alone. That extra mass is dark matter.
Astronomy: Roen Kelly, after Mario De Leo/Wikimedia Commons